Gene description for HLA-DRA
Gene name major histocompatibility complex, class II, DR alpha
Gene symbol HLA-DRA
Other names/aliases HLA-DRA1
Species Homo sapiens
 Experiment description of studies that identified HLA-DRA in B cells

1
Experiment ID11
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 12519789   
OrganismHomo sapiens
Experiment descriptionProteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
AuthorsWubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mös W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W
Journal name JBC
Publication year2003
SampleB cells
Sample nameRN (HLA-DR15+)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.15 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
High performance liquid chromatography
EV-TRACK -

2
Experiment ID37
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 10878338   
OrganismHomo sapiens
Experiment descriptionDirect immunosuppressive effects of EBV-encoded latent membrane protein 1.
AuthorsDukers DF, Meij P, Vervoort MB, Vos W, Scheper RJ, Meijer CJ, Bloemena E, Middeldorp JM
Journal name JIMMU
Publication year2000
SampleB cells
Sample nameJY
B95-8
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

3
Experiment ID47
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method FACS
PubMed ID 17641064   
OrganismHomo sapiens
Experiment descriptionExosomes with immune modulatory features are present in human breast milk.
AuthorsAdmyre C, Johansson SM, Qazi KR, FiléJJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year2007
SampleB cells
Sample nameB cells
Isolation/purification methodsDifferential centrifugation
Filtration
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
EV-TRACK -

4
Experiment ID79
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%

5
Experiment ID80
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%

6
Experiment ID81
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%

7
Experiment ID308
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry [MALDI TOF/TOF]
Mass spectrometry [Q-TOF]
PubMed ID 16342139   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of malignant lymphocyte membrane microparticles using double ionization coverage optimization
AuthorsMiguet L, Pacaud K, Felden C, Hugel B, Martinez MC, Freyssinet JM, Herbrecht R, Potier N, van Dorsselaer A, Mauvieux L
Journal name Proteomics
Publication year2006
SampleB cells
Sample nameB cells - Chronic B cell lymphoid patient
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [MALDI TOF/TOF]
Mass spectrometry [QTOF]
EV-TRACK -

8
Experiment ID395
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 19413345   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of malignant B-cell derived microparticles reveals CD148 as a potentially useful antigenic biomarker for mantle cell lymphoma diagnosis.
AuthorsMiguet L, Béchade G, Fornecker L, Zink E, Felden C, Gervais C, Herbrecht R, Van Dorsselaer A, Mauvieux L, Sanglier-Cianferani S.
Journal name J Proteome Res
Publication year2009
SampleB cells
Sample namePatients of chronic lymphocytic leukemia-B cells
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

9
Experiment ID396
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 19413345   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of malignant B-cell derived microparticles reveals CD148 as a potentially useful antigenic biomarker for mantle cell lymphoma diagnosis.
AuthorsMiguet L, Béchade G, Fornecker L, Zink E, Felden C, Gervais C, Herbrecht R, Van Dorsselaer A, Mauvieux L, Sanglier-Cianferani S.
Journal name J Proteome Res
Publication year2009
SampleB cells
Sample namePatients of mantle cell lymphoma-B cells
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

10
Experiment ID397
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 19413345   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of malignant B-cell derived microparticles reveals CD148 as a potentially useful antigenic biomarker for mantle cell lymphoma diagnosis.
AuthorsMiguet L, Béchade G, Fornecker L, Zink E, Felden C, Gervais C, Herbrecht R, Van Dorsselaer A, Mauvieux L, Sanglier-Cianferani S.
Journal name J Proteome Res
Publication year2009
SampleB cells
Sample namePatients of small cell lymphoma-B cells
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

11
Experiment ID534
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles/Oncosomes/Microparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23818640   
OrganismHomo sapiens
Experiment descriptionModulation of B-cell exosome proteins by gamma herpesvirus infection.
AuthorsMeckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N.
Journal name Proc Natl Acad Sci U S A
Publication year2013
SampleB cells
Sample nameEBV- and KSHV-infected B cells (JSC-1, BC1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130064: EV-METRIC:25%

12
Experiment ID535
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles/Oncosomes/Microparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23818640   
OrganismHomo sapiens
Experiment descriptionModulation of B-cell exosome proteins by gamma herpesvirus infection.
AuthorsMeckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N.
Journal name Proc Natl Acad Sci U S A
Publication year2013
SampleB cells
Sample nameEBV-infected B cells (#1, HLJ, IM9, CP)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130064: EV-METRIC:25%

13
Experiment ID536
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles/Oncosomes/Microparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23818640   
OrganismHomo sapiens
Experiment descriptionModulation of B-cell exosome proteins by gamma herpesvirus infection.
AuthorsMeckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N.
Journal name Proc Natl Acad Sci U S A
Publication year2013
SampleB cells
Sample nameKSHV-infected B cell (JC, BC3, BCP1, BCBL1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130064: EV-METRIC:25%

14
Experiment ID537
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles/Oncosomes/Microparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23818640   
OrganismHomo sapiens
Experiment descriptionModulation of B-cell exosome proteins by gamma herpesvirus infection.
AuthorsMeckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N.
Journal name Proc Natl Acad Sci U S A
Publication year2013
SampleB cells
Sample nameNormal B cells (BJAB)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130064: EV-METRIC:25%

15
Experiment ID545
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23983189   
OrganismHomo sapiens
Experiment descriptionCharacterization of Multiple Myeloma Vesicles by Label-Free Relative Quantitation.
AuthorsHarshman SW, Canella A, Ciarlariello PD, Rocci A, Agarwal K, Smith EM, Talabere T, Efebera YA, Hofmeister CC, Benson DM Jr, Paulaitis ME, Freitas MA, Pichiorri F.
Journal name Proteomics
Publication year2013
SampleB cells
Sample nameMM.1S
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130092: EV-METRIC:22%

16
Experiment ID546
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23983189   
OrganismHomo sapiens
Experiment descriptionCharacterization of Multiple Myeloma Vesicles by Label-Free Relative Quantitation.
AuthorsHarshman SW, Canella A, Ciarlariello PD, Rocci A, Agarwal K, Smith EM, Talabere T, Efebera YA, Hofmeister CC, Benson DM Jr, Paulaitis ME, Freitas MA, Pichiorri F.
Journal name Proteomics
Publication year2013
SampleB cells
Sample nameU266
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130092: EV-METRIC:22%

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